Search results for " alpha-enolase"

Myc Promoter-Binding Protein-1 (MBP-1) Is a Novel Potential Prognostic Marker in Invasive Ductal Breast Carcinoma

2010

BackgroundAlpha-enolase is a glycolytic enzyme that catalyses the formation of phosphoenolpyruvate in the cell cytoplasm. α-Enolase and the predominantly nuclear Myc promoter-binding protein-1 (MBP-1) originate from a single gene through the alternative use of translational starting sites. MBP-1 binds to the P2 c-myc promoter and competes with TATA-box binding protein (TBP) to suppress gene transcription. Although several studies have shown an antiproliferative effect of MBP-1 overexpression on several human cancer cells, to date detailed observations of α-enolase and MBP-1 relative expression in primary tumors versus normal tissues and their correlation with clinicopathological features ha…

An integrated humoral and cellular response is elicited in pancreatic cancer by alpha-enolase, a novel pancreatic ductal adenocarcinoma-associated an…

2009

Pancreatic ductal adenocarcinoma (PDAC) is a fatal disease with a very poor 5-year survival rate. alpha-Enolase is a glycolytic enzyme that also acts as a surface plasminogen receptor. We find that it is overexpressed in PDAC and present on the cell surface of PDAC cell lines. The clinical correlation of its expression with tumor status has been reported for lung and hepatocellular carcinoma. We have previously demonstrated that sera from PDAC patients contain IgG autoantibodies to alpha-enolase. The present work was intended to assess the ability of alpha-enolase to induce antigen-specific T cell responses. We show that alpha-enolase-pulsed dendritic cells (DC) specifically stimulate healt…

Role of Enolase/MBP-1 in non-tumorigenic and cancer cells

The glycolytic enzyme α-enolase is a highly conserved protein involved in multiple functions (Díaz-Ramos A et al 2012). Besides the mainly cytoplasmic localization, the protein has been detected on the surface of prokaryotic and eukaryotic cells where it functions as a plasminogen receptor, while a shorter variant, called Myc promoter-binding protein-1 (MBP-1), is mainly located in the nucleus. Several lines of evidence indicate that MBP-1 acts as a tumor suppressor, negatively regulating cell proliferation or promoting apoptosis of cancer cells. Although a few reports indicate that stressful conditions, such as glucose deprivation or hypoxia, may modulate MBP-1 expression in mammalian cell…

Natural bioactive molecules induce a reduction of surface alpha-enolase in breast cancer cells

Cell surface expression of alpha-enolase, a glycolytic enzyme displaying moonlighting activities, has been shown to contribute to cancer cell invasion and metastasis formation. Although the functional role of surface alpha-enolase in cancer spreading has been clearly linked to the protein non-enzymatic function of binding plasminogen and enhancing plasmin formation, the cellular pathways underlying cell surface transport remain largely elusive. We have previously demonstrated that pro-invasive stimuli promote the surface expression of alpha-enolase in breast cancer cells. To further investigate alpha-enolase translocation to the plasma membrane and to identify the signaling involved in this…